Absolute quantification of cassava brown streak virus mRNA by real-time qPCR
نویسندگان
چکیده
منابع مشابه
Absolute quantification of cassava brown streak virus mRNA by real-time qPCR
Cassava brown streak disease (CBSD) is the most important virus disease of cassava and a major food security threat in Africa. Yearly economic losses of up to $100 million USD have been attributed to CBSD. The lack of information on plant-virus interactions has restricted progress in breeding for CBSD resistance. Virus quantification is becoming a major tool for the quick and reliable assessmen...
متن کاملAdvances in cassava research in management of Cassava Brown Streak Virus Disease in Uganda
Résumé Research Application Summary Advances in cassava research in management of Cassava Brown Streak Virus
متن کاملOptimisation of in Vitro Techniques for Cassava Brown Streak Virus Elimination from Infected Cassava Clones
Cassava brown streak disease (CBSD), caused by Cassava brown streak virus (CBSV), is an economically important disease of cassava (Manihot esculenta Crantz) in East Africa. The objective of this study was to optimise in vitro techniques for CBSV elimination from infected Ugandan cassava cultivars. Using semi-solid halfstrength Murashige and Skoog (MS) basal medium, hormone concentration and hea...
متن کاملCytokine mRNA quantification by real-time PCR.
Real-time PCR represents a new methodology that accurately quantifies nucleic acids. This has been made possible by the use of fluorogenic probes, which are presented in two forms, namely hydrolysis probes (also called TaqMan probes) and hybridisation probes. We decided to apply this methodology to cytokine mRNA quantification and this led us to the development of a protocol that provides an ea...
متن کاملHigh-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants.
AIMS To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. METHODS AND RESULTS The diagnostic assay was developed for two RNA viruses; Cassava brown streak virus (CBSV) and Uganda cassava brown streak virus (UCBSV) and two predominant DNA viruses; Afr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2017
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2017.03.003